Rat islet isolation



Keywords: rat islet isolation
Description: Islet-cell transplantation appears to be a fast-growing new subject in the field of experimental research, as a look at the literature shows. There may be two main reasons for this expanding

Islet-cell transplantation appears to be a fast-growing new subject in the field of experimental research, as a look at the literature shows. There may be two main reasons for this expanding interest: (1) Isolated islets, provided they are clean, undamaged, and numerous, can cure artifically induced diabetes in rats and mice. This means that islet transplantation must be regarded as a potential therapy protocol for the treatment of diabetes in man as well. (2) Isolated islets provide the immunobiologist with an ideal microtissue to analyze and to manipulate in vitro, which is a chance second to none to overcome the problem of “tissue immunogenicity,” regarded as one major barrier in both experimental and clinical transplantation. Whatever the basic interests are, one needs islets of high quality and sufficient quantity. This article aims to draw the reader’s attention to only a few, but vital, technical points concerning isolation, cleaning, and transplantation of islets. Some of the details here may have already been analyzed thoroughly by others and written down elsewhere. These lines reflect the personal views, problems, and experiences of newcomers in the field of experimental islet transplantation.






Photogallery Rat islet isolation:


Significance of the peri-insular extracellular matrix for islet ...


Optimizing Conditions for Rat Pancreatic Islets Isolation - Springer


Isolation and characterisation of insulin secretory granules from ...


Effects of noradrenaline on45Ca2+ efflux from isolated rat islets ...


Effects of islet amyloid polypeptide on hepatic insulin resistance ...


An analysis of the role of collagenase and protease in the ...


Experimental studies on cryopreservation combined with the ...


Isolation and purification of rat islet cells by flow cytometry ...


Experiences in Rat Pancreatic Islet-Cell Isolation and ...


Stimulation of insulin release in isolated rat islets by GIP in ...


In vivo assessment of isolated pancreatic islet viability using ...


Isolation and long term preservation of pancreatic islets from ...


Enterostatin inhibits insulin secretion from isolated perifused ...


Permissive effect of glucose on the glucagon-induced accumulation ...


Studies on glucagon secretion using isolated islets of langerhans ...


Studies on glucagon secretion using isolated islets of langerhans ...


Tritiated taurine handling by isolated rat pancreatic islets ...


The c-Jun N-terminal kinase JNK participates in cytokine- and ...