Pereira smith



Keywords: aging, airway defense, androgen action, androgen receptor, animal models, apoptosis, autoimmunity, breast cancer, calcium, calcium atpases, calcium binding proteins, cancer, cell adhesion proteins, cell biology, central nervous system, chromosome 18, colon cancer, comparative cytogenetics, development, dna repair, dopamine, education, endocrinology, excitable medium, fluorescence in situ hybridization fish, free radicals, gene expression, gene requlation, genetics, gonadotrophic hormones, histology, hormone action, hypoxia, immunology, iron metabolism, karyotyping, lung, map kinase pathway, metastasis, mitochondria, molecular, mutagenesis, neurobiology, neurodegenerative diseases, neuroendocrinology, nutrition, omega 3 polyunsaturated fatty acids, optical imaging, oxidative injury, oxidative stress, paget disease of the bone, pain, parkinson's disease, periodontitis, pituitary gland, prostate, prostate cancer, reproductive biology, reproductive physiology, spermatogenic cell types, steroid receptors, tissue engineering, transcription, transcription factors, transgenic mice, tumor suppressor gene, tyrosine hydroxylase, virtual reality, xenopus oocytes,phd, masters, md, phd training, nathan shock center for aging, nih, diabetes, cancer, aids, stroke preventionn, aging, basic biomedical science, drug development, pediatric cancer research, howard hughes medical institute, national cancer institute patient trials, cell biology, molecular biology, biotechnology, imaging core, bioinformatics core, transgenic core, research, optical microscopy
Description: Faculty of the Department of Cellular and Structural Biology - The University of Texas Health Science Center at San Antonio. Provides instruction for the Schools of Medicine, Dentistry, Allied Health, Nursing and the Graduate School, and is consistently ranked in the top ten of all cell/molecular biology departments in the nation for total NIH funding

The limited division of normal cells in culture is proposed as a model for cell aging. To understand the molecular and genetic basis for this loss of cell division in normal cells, we have chosen to study abnormal human cells (tumor derived, virus transformed) that have escaped from senescence and proliferate indefinitely (immortal). We have found that hybrids from fusion of various immortal cells with normal cells regain the senescent phenotype. This indicates that the phenotype of immortality is the result of recessive changes in genes regulating growth. We then fused various immortal human cells with each other. If the cell lines had immortalized through the same changes, immortal hybrids would be obtained. If different events had led to immortalization, hybrids would have limited division since the recessive (defective) genes of each cell line would be complemented by the good genes present in the other.

By this analysis we have identified four complementation groups for indefinite division. Using microcell fusion we determined that a normal human chromosome 4 can suppress the immortal phenotype in immortal cells lines assigned to group B. We then cloned the gene MORF4 as the cause of this effect. It is a member of a family of genes that are involved in chromatin remodeling and transcriptional regulation.

The MORF related genes, MRG15 and MRGX encode proteins that are present in multiple nucleoprotein complexes and the composition of other proteins in these complexes determines whether a gene promoter is activated or repressed. MRG15 has now been implicated in chromatin remodeling. It is involved in transcriptional regulation essential for cell proliferation, DNA repair, regulation of translation from RNA to protein and also RNA splicing via histone code recognition. Studies with neural stem/progenitor cells derived from brains of MRG15 null and wild type embryos and the initial published results indicate that null cells have defects in both proliferation and differentiation into neurons but not glial cells.

We are also involved in collaborative studies on telomeres and telomerase with Drs. I Rubelj, V Gorbunova and A Seluanov, and on mortalin with Drs R Wadhwa and S Kaul.

Cell culture of normal and immortal human and mouse cells, use of genetically modified mice, immunostaining and histochemistry, molecular and biochemical techniques: Southern, northern, western, immunoprecipitation, chromatin immunoprecipitation, PCR, real time PCR, plasmid constructs, adenoviral constructs.






Photogallery Pereira smith:


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